pcfj90 (Addgene inc)
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pcfj90
Pcfj90, supplied by Addgene inc, used in various techniques. Bioz Stars score: 95/100, based on 173 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pcfj90+pmyo/pCFJ90+-+Pmyo-2%3A%3AmCherry%3A%3Aunc-54utr+(Plasmid+%2319327)/bio_rxiv__2025__11__24__690166-389-20-26
Average 95 stars, based on 173 article reviews
Pcfj90, supplied by Addgene inc, used in various techniques. Bioz Stars score: 95/100, based on 173 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pcfj90+pmyo/pCFJ90+-+Pmyo-2%3A%3AmCherry%3A%3Aunc-54utr+(Plasmid+%2319327)/bio_rxiv__2025__11__24__690166-389-20-26
Average 95 stars, based on 173 article reviews
pcfj90 - by Bioz Stars,
2026-10
95/100 stars
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Plasmid Preparation:Article Title: Lysosome-related organelles contain an expansion compartment that mediates delivery of zinc transporters to promote homeostasis. Article Snippet: .. We thank Laura Kyro for graphics and Suzanne Pfeffer for advice. Article Title: The Caenorhabditis elegans HAM-1 protein modifies G protein signaling and membrane extension to reverse the polarity of asymmetric cell division Article Snippet: Similarly, pENTR Pmab-5 and pENTR 3’unc-54 entry clones were created by PCR-amplification of the 8.8 kb mab-5 promoter and the unc-54 3’UTR, respectively, and Gateway cloning into pDONR P4-P1R and pDONR P2R-P3 (Invitrogen), respectively. .. A multisite 4-1-2-3 Gateway reaction was used to create Pmab-5::gfp::ham-1 from these three entry clones and the destination vector pDEST R4R3 (Invitrogen).The gmEx699 and gmEx701 transgenes were created using standard procedures by coinjecting the Pmab-5:gfp::ham-1 plasmid at 25ng/μl and the Article Title: Novel signaling mechanisms downstream of NIPI-3/Tribbles regulate development in Caenorhabditis elegans Article Snippet: .. A mixture of 20 ng/μl of sgRNA, 80 ng/μl of homology 82 arm repair template, and 2.5 ng/μl of Article Title: Disrupted Autophagy and Neuronal Dysfunction in C. elegans Knock-in Models of FUS Amyotrophic Lateral Sclerosis Article Snippet: .. To generate animals expressing GFP::FUST-1 in cholinergic motor neurons, Punc-17::GFP::fust-1::unc-54 3’ UTR plasmids carrying wild type or mutant fust-1 allele were injected at 25 ng/ul into non-transgenic N2 animals with 100 ng/ul of pBlueScript vector and 2.5 ng/ul of Article Title: Microbial Siderophore Enterobactin Promotes Mitochondrial Iron Uptake and Development of the Host via Interaction with ATP Synthase. Article Snippet: .. CGC WB Strain: XA6901 C. elegans: Strain: SJ4103: zcIs14 [myo-3::GFP(mit)] CGC WB Strain: SJ4103 C. elegans: Ex[rpl-28P:atp-1(deletion residues 198-205: DRQTGKTA):unc-54 30UTR], atp-1(lf) This study N/A Oligonucleotides Primers (see Table S2) This paper N/A ATP5A1 siRNA (see Table S2) Sigma Cat #NM_001001937 Recombinant DNA Plasmid: pPD95.77 pPD95.77 was a gift from Andrew Fire Addgene Plasmid #1495 Plasmid: pET30a Merck Millipore, Novagen Cat#69909 Plasmid: Article Title: Centriolar SAS-7 acts upstream of SPD-2 to regulate centriole assembly and pericentriolar material formation Article Snippet: The carrier used was HB101 E. coli DNA purified using a GeneElute Bac- terial Genomic DNA kit (Sigma-Aldrich, St. Louis, MO), which was then digested with PvuII and puri- fied on a QiaQuick column (Qiagen). .. The linearized markers for transformation used were Plasmid 19327: Article Title: Forward genetic screening identifies novel roles for N-terminal acetyltransferase C and histone deacetylase in C. elegans development Article Snippet: .. A mixture of 20 ng/μl of sgRNA, 80 ng/μl of homology arm repair template, and 2.5 ng/μl of Article Title: Peel-1 negative selection promotes screening-free CRISPR-Cas9 genome editing in Caenorhabditis elegans Article Snippet: .. Peel-1-DMS injection mixes were prepared the same except that they included pMA122 ( Phsp16.41∷peel-1 ) at 10ng/μl for peel-1 negative selection ( ). Clone Assay:Article Title: The Caenorhabditis elegans HAM-1 protein modifies G protein signaling and membrane extension to reverse the polarity of asymmetric cell division Article Snippet: Similarly, pENTR Pmab-5 and pENTR 3’unc-54 entry clones were created by PCR-amplification of the 8.8 kb mab-5 promoter and the unc-54 3’UTR, respectively, and Gateway cloning into pDONR P4-P1R and pDONR P2R-P3 (Invitrogen), respectively. .. A multisite 4-1-2-3 Gateway reaction was used to create Pmab-5::gfp::ham-1 from these three entry clones and the destination vector pDEST R4R3 (Invitrogen).The gmEx699 and gmEx701 transgenes were created using standard procedures by coinjecting the Pmab-5:gfp::ham-1 plasmid at 25ng/μl and the Injection:Article Title: Novel signaling mechanisms downstream of NIPI-3/Tribbles regulate development in Caenorhabditis elegans Article Snippet: .. A mixture of 20 ng/μl of sgRNA, 80 ng/μl of homology 82 arm repair template, and 2.5 ng/μl of Article Title: Disrupted Autophagy and Neuronal Dysfunction in C. elegans Knock-in Models of FUS Amyotrophic Lateral Sclerosis Article Snippet: .. To generate animals expressing GFP::FUST-1 in cholinergic motor neurons, Punc-17::GFP::fust-1::unc-54 3’ UTR plasmids carrying wild type or mutant fust-1 allele were injected at 25 ng/ul into non-transgenic N2 animals with 100 ng/ul of pBlueScript vector and 2.5 ng/ul of Article Title: Forward genetic screening identifies novel roles for N-terminal acetyltransferase C and histone deacetylase in C. elegans development Article Snippet: .. A mixture of 20 ng/μl of sgRNA, 80 ng/μl of homology arm repair template, and 2.5 ng/μl of Article Title: Peel-1 negative selection promotes screening-free CRISPR-Cas9 genome editing in Caenorhabditis elegans Article Snippet: .. Peel-1-DMS injection mixes were prepared the same except that they included pMA122 ( Phsp16.41∷peel-1 ) at 10ng/μl for peel-1 negative selection ( ). Expressing:Article Title: Disrupted Autophagy and Neuronal Dysfunction in C. elegans Knock-in Models of FUS Amyotrophic Lateral Sclerosis Article Snippet: .. To generate animals expressing GFP::FUST-1 in cholinergic motor neurons, Punc-17::GFP::fust-1::unc-54 3’ UTR plasmids carrying wild type or mutant fust-1 allele were injected at 25 ng/ul into non-transgenic N2 animals with 100 ng/ul of pBlueScript vector and 2.5 ng/ul of Mutagenesis:Article Title: Disrupted Autophagy and Neuronal Dysfunction in C. elegans Knock-in Models of FUS Amyotrophic Lateral Sclerosis Article Snippet: .. To generate animals expressing GFP::FUST-1 in cholinergic motor neurons, Punc-17::GFP::fust-1::unc-54 3’ UTR plasmids carrying wild type or mutant fust-1 allele were injected at 25 ng/ul into non-transgenic N2 animals with 100 ng/ul of pBlueScript vector and 2.5 ng/ul of Western Blot:Article Title: Microbial Siderophore Enterobactin Promotes Mitochondrial Iron Uptake and Development of the Host via Interaction with ATP Synthase. Article Snippet: .. CGC WB Strain: XA6901 C. elegans: Strain: SJ4103: zcIs14 [myo-3::GFP(mit)] CGC WB Strain: SJ4103 C. elegans: Ex[rpl-28P:atp-1(deletion residues 198-205: DRQTGKTA):unc-54 30UTR], atp-1(lf) This study N/A Oligonucleotides Primers (see Table S2) This paper N/A ATP5A1 siRNA (see Table S2) Sigma Cat #NM_001001937 Recombinant DNA Plasmid: pPD95.77 pPD95.77 was a gift from Andrew Fire Addgene Plasmid #1495 Plasmid: pET30a Merck Millipore, Novagen Cat#69909 Plasmid: pCFJ90 - Pmyo-2::mCherry::unc-54utr (Frøkjaer-Jensen et al., 2008) Addgene Plasmid Plasmid #19327 Plasmid: rpl-28P:atp-1(deletion residues 198-205: DRQTGKTA):unc-54 30UTR This paper N/A Software and Algorithms Fiji (ImageJ) http://fiji.sc Ver 1.50i; RRID:SCR_002285 WormSizer (Moore et al., 2013) https://github.com/bradtmoore/wormsizer Please cite this article in press as: Qi and Han, Microbial Siderophore Enterobactin Promotes Mitochondrial Iron Uptake and Development of the Host via Interaction with ATP Synthase, Cell (2018), https://doi.org/10.1016/j.cell.2018.07.032 .. Recombinant:Article Title: Microbial Siderophore Enterobactin Promotes Mitochondrial Iron Uptake and Development of the Host via Interaction with ATP Synthase. Article Snippet: .. CGC WB Strain: XA6901 C. elegans: Strain: SJ4103: zcIs14 [myo-3::GFP(mit)] CGC WB Strain: SJ4103 C. elegans: Ex[rpl-28P:atp-1(deletion residues 198-205: DRQTGKTA):unc-54 30UTR], atp-1(lf) This study N/A Oligonucleotides Primers (see Table S2) This paper N/A ATP5A1 siRNA (see Table S2) Sigma Cat #NM_001001937 Recombinant DNA Plasmid: pPD95.77 pPD95.77 was a gift from Andrew Fire Addgene Plasmid #1495 Plasmid: pET30a Merck Millipore, Novagen Cat#69909 Plasmid: Software:Article Title: Microbial Siderophore Enterobactin Promotes Mitochondrial Iron Uptake and Development of the Host via Interaction with ATP Synthase. Article Snippet: .. CGC WB Strain: XA6901 C. elegans: Strain: SJ4103: zcIs14 [myo-3::GFP(mit)] CGC WB Strain: SJ4103 C. elegans: Ex[rpl-28P:atp-1(deletion residues 198-205: DRQTGKTA):unc-54 30UTR], atp-1(lf) This study N/A Oligonucleotides Primers (see Table S2) This paper N/A ATP5A1 siRNA (see Table S2) Sigma Cat #NM_001001937 Recombinant DNA Plasmid: pPD95.77 pPD95.77 was a gift from Andrew Fire Addgene Plasmid #1495 Plasmid: pET30a Merck Millipore, Novagen Cat#69909 Plasmid: Transformation Assay:Article Title: Centriolar SAS-7 acts upstream of SPD-2 to regulate centriole assembly and pericentriolar material formation Article Snippet: The carrier used was HB101 E. coli DNA purified using a GeneElute Bac- terial Genomic DNA kit (Sigma-Aldrich, St. Louis, MO), which was then digested with PvuII and puri- fied on a QiaQuick column (Qiagen). .. The linearized markers for transformation used were Plasmid 19327: Selection:Article Title: Peel-1 negative selection promotes screening-free CRISPR-Cas9 genome editing in Caenorhabditis elegans Article Snippet: .. Peel-1-DMS injection mixes were prepared the same except that they included pMA122 ( Phsp16.41∷peel-1 ) at 10ng/μl for peel-1 negative selection ( ). |